Microbiology- What was the population density of the original sample

Student Name: Click here to enter text.

Kit Code (located on the lid of your lab kit): Click here to enter text.

“Experiment 1- Direct Counts Following Serial Dilution”

“Table 1: Experiment 1 Growth Results”

“Plate”	“Classification”	“CFU/plate”
“10-1”	Click here to enter text.	Click here to enter text.
“10-2”	Click here to enter text.	Click here to enter text.
“10-3”	Click here to enter text.	Click here to enter text.
“10-4”	Click here to enter text.	Click here to enter text.
“10-5”	Click here to enter text.	Click here to enter text.
“10-6”	Click here to enter text.	Click here to enter text.

“Post-Lab Questions”

“1. What was the population density of the original sample? What would have happened if you had inoculated an agar plate with 1mL of the original sample?”

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“2. Did all the bacterial colonies on the countable plate(s) have a similar appearance? If not, how would you explain this?”

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“3. You have performed a serial dilution of an unknown sample and counted 73 CFU on a countable plate that was marked as a 10^-4 dilution and you used 0.1mL to inoculate the plate. What is the population density of the original sample?”

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“4. List at least one advantage and one disadvantage to the direct plate counting method following serial dilution for determining bacterial concentration.”

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“5. Compare and contrast direct plate counts for bacterial and viral populations.”

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“6. What are some sources of error in the serial dilution/direct plate counting method?”

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“Insert photo of your cultures after incubation with your name clearly visible in the background:”