Lab 7 Differential stains – Acid fast stain

Lab 7 – Differential stains – Acid fast stain

Title:

Purpose:

Methods: Lab book page 195 - 198 video observations,

https://www.youtube.com/watch?v=faB_5STfZH8

List the steps for setting up the slide and preforming an acid-fast stain

https://www.cdc.gov/DiseasesConditions/

to get information for answering questions in the conclusion

 

Results observed:

Fill in the results in the table and then write the conclusion

Bacteria name	Acid-Fast positive or negative	Disease caused by this bacteria
Mycoplasma pneumoniae
Mycobacterium leprae
Shigella dysenteriae
Mycobacterium tuberculosis
Chlamydia trachomatis

 

 

 

Conclusion:

In a narrative form

What molecule do the acid-fast bacteria have in their cell wall that “makes” them acid-fast?

What dye is used to stain the cells that are positive in this reaction? What color would the positive cells stain? What dye is used as the counterstain in this reaction? What color would the negative cells stain?

 

An acid-fast stain is used to visualize bacteria belonging to which Genus?

Which bacteria of this genus causes tuberculosis? How many cases of TB were diagnosed in the US in 2019? How many people are living with TB in the United States in 2019?

How is the drug regime to treat a case of active drug-susceptible TB (intensive phase and continuation phase)? What are the antibiotics used?

 

Which bacteria of this genus causes leprosy? Approximately how many cases of leprosy are diagnosed in the US each year? How long does it take to treat a case of leprosy? What are the antibiotics used?