Beer’s Law Assignment
Title: Beer’s Law
Purpose:
Procedure:
Data Tables:
Data Table 1: Sample Preparations
TUBE LABEL | VOLUME OF DISTILLED WATER (mL) | VOLUME OF STANDARD BLUE DYE (mL) | TOTAL VOLUME IN TEST TUBE (mL) | FINAL CONCENTRATION OF BLUE DYE IN TEST TUBE (M) |
B | 0mL | 5mL | 5.0 | 2.57x10-4M |
1 | 0.5 mL | 4.5 mL | 5.0 | |
2 | 1.0 mL | 4.0 mL | 5.0 | |
3 | 1.5 mL | 3.5 mL | 5.0 | |
4 | 2.0 mL | 3.0 mL | 5.0 | |
5 | 2.5 mL | 2.5 mL | 5.0 | |
6 | 3.0 mL | 2.0 mL | 5.0 | |
7 | 3.5 mL | 1.5 mL | 5.0 | |
8 | 4.0 mL | 1.0 mL | 5.0 | |
9 | 4.5 mL | 0.5 mL | 5.0 | |
W | 5.0 mL | 0 mL | 5.0 | 0 M |
Data Table 2: Concentration and Resistance
TUBE LABEL | CONCENTRATION OF BLUE DYE IN TEST TUBE (M) | RESISTANCE READING ON COLORIMETER (?) |
B | 2.57x 10-4M | |
1 | ||
2 | ||
3 | ||
4 | ||
5 | ||
6 | ||
7 | ||
8 | ||
9 | ||
W | 0 M | |
CD 1 | ||
CD 2 |
Graph 1: Plot of Concentration vs. Resistance
PLOT |
Questions:
A. Describe possible sources of error in this experiment.
The remainder of these questions are based upon the following scenario:
A testing laboratory has been hired by a company called “Drug Company Q” to analyze a series of over-the-counter drugs that the company produces. In these over-the-counter drugs, the active ingredient is called “Active Ingredient M.” The laboratory technician collected the following data from samples with known concentrations of the Active Ingredient M. That data is shown below in Table 1.
Table 1.Known concentration of M drugs
Sample Identification Code | Sample Concentration (M) | Absorbance |
Q5000 | 4.00 x 10-4 | 17.9 |
Q5001 | 3.20 x 10-4 | 25 |
Q5002 | 2.40 x 10-4 | 35.7 |
Q5003 | 1.60 x 10-4 | 50.2 |
Q5004 | 8.00 x 10-5 | 70.8 |
The technician also collected absorbance readings for the 5 over-the-counter drugs for review. The data collected for the 5 over-the-counter drugs is shown in Table 2.
Table 2.Absorbance data for over-the-counter drugs
Sample Identification Code | Absorbance |
M21050-1 | 43.7 |
M21050-2 | 44.1 |
M21050-3 | 45.8 |
M21050-4 | 42.1 |
M21050-5 | 30.1 |
B. Create a Beer’s law plot and best fit line for the data in Table 1.
C. Use the Beer’s law plot and best fit line to determine the concentrations for samples: M21050-1, M21050-2, M21050-3, M21050-4, M21050-5.
D. The company reported that sample M21050-2 has an M concentration of 0.0003M. Assuming that the results in Question C are 100% accurate and without error, is the company’s statement accurate? What is the percent error between the reported concentration and the concentration calculated in Question C?
E. By law, Drug Company Q must have an M concentration between 2.85 x 10-4M and 3.15 x 10-4M. Do all samples analyzed meet the legal requirements? Use the information from Question C to explain your answer.
Conclusion:
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Rating:
/5
Solution: Beer’s Law Assignment