Conduction of protons by the F0 unit of ATP synthase
Question # 00795411
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Updated on: 02/23/2021 10:14 AM Due on: 02/23/2021
Site directed mutagenesis can be used to study ATP synthase
Conduction of protons by the F0 unit of ATP synthase is blocked by modification of a single side chain by dicyclohexylcarboiimide (DCCD). What are the most likely targets of action of this reagent? How might you use site-directed mutagenesis to determine whether this residue is essential for proton conduction?
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Solution: Conduction of protons by the F0 unit of ATP synthase