BIOLOGY 1510 - The glow-in-the-dark trait gene

Post-laboratory Questions: Need some help with Gene splicing lab questions.

1. The glow-in-the-dark trait gene:

a. Comes from a firefly

b. Comes from E. coli

c. Could be used in anti-HIV drug development

d. A and C

2. Antifreeze plasma proteins:

a. Have been isolated in species of flounder

b. Allow some species of fish to grow at very hot temperatures

c. Allow some species of fish to grow at very cold temperatures

d. A and C

e. All of the above

3. Insulin:

a. Can be produced in genetically engineered bacteria

b. Can be produced in cloned cows

c. Can be produced more cheaply in cow's milk

d. B and C

e. All of the above

4. The rot-resistant trait:

a. Was introduced into the Flavr Savr tomato

b. Is due to antisense suppression of polygalacturonase

c. Increased the cost of processed tomato products such as tomato paste

d. A and B

5. Genes have NOT been successfully introduced ("spliced") into:

a. Prokaryotes

b. Plants

c. Animals

d. None of the above

6. Restriction enzymes:

a. Were originally isolated from bacteria

b. Only cut DNA at random sequences

c. Can cut DNA at specific sequences

d. Always leave blunt ends

e. A and C

7. A proper Watson-Crick DNA base pair is:

a. A-G

b. C-T

c. A-T

d. None of the above

8. "Sticky ends" allow DNA fragments to be spliced into vectors by what type of bonding?

a. Ionic

b. Covalent

c. Hydrogen

d. None of the above

9. The enzyme ligase:

a. Cuts DNA and creates blunt ends

b. Cuts DNA and creates sticky ends

c. Completes the splicing of DNA fragments and plasmid vectors

d. None of the above

10. You cut a vector with the following DNA base overhang ("sticky end"):

ACCTGGACTTA

CCTGAAT

You will need to cut out the DNA fragment containing your gene of interest so that which sticky end is created?

a. XXXXXXXX

XXXXXXXXTGGA

b. XXXXXXXX

XXXXXXXXACCT

c. XXXXXXXX

XXXXXXXXTCCA

d. XXXXXXXX

XXXXXXXXATTC