BIOL350 project 3

Project 3: Characterizing Genes (An Experimental Design Project)

Addresses course outcome 2:

Select and apply knowledge of cellular and molecular processes and current laboratory techniques to effectively participate in the design of scientific studies

The goal of this group project is to develop a mouse model system for studying how mutations in the murine (mouse) IGF-1 gene might impact body size and lifespan in the mouse. (To learn about IGF-1 and how it functions to regulate growth, longevity, and cancer susceptibility, see the review I have posted in the conference titled "Group Project Reading Material." Also in this conference, you will find other resources related to molecular and cellular biology techniques.)

As part of this project, your goals are to:

1-Clone the mouse IGF-1 gene.

2-Characterize tissue expression patterns of mouse IGF-1 mRNA and protein.

3-Introduce mutations into the cloned IGF-1 gene, and determine whether these mutations affect expression of the gene*.

4-Generate an IGF-1 "knockin" mouse. Here, you will "knockin" your mutated IGF-1 gene into the mouse IGF-1 locus so that you can finally determine whether the mutations you have made in the gene will affect mouse size and lifespan.

*Any of the assays you work out to monitor expression of the normal mouse IGF-1 gene can then be used to assess expression of the mutated versions of the gene that you will be constructing.??

During the course of the semester each group will post their progress on each goal (1 - 4 above) in the appropriate conference area. This will allow everyone to read and discuss the ideas in progress. This process is meant to enhance each groups project and come up with the best solutions to our challenge of characterizing IGF-1 in the normal mouse and the “knock-in” mouse.

Each member of the group is encouraged to participate in the conference by putting forth his or her ideas relative to other groups' progress and implementation of their plan. You may ask questions of each other and give suggestions, and I will also ask questions or give advice as we proceed.

The final product, including all goals and how they will be accomplished will be due at the end of the semester. This paper should be no longer than 4 pages (single spaced, 12pt font) and submitted to the Assignment folder. I only need one copy, so each group can appoint one group member to submit the final draft.