BIO Lab 20 Pre-Lab Questions (2015)
Experiment 1: Paper Chromatography
In this experiment, you will separate plant pigments using chromatography. You will also measure the rate of photosynthesis in isolated chloroplasts using the reduction of the dye 2,6-Dichloroindophenol (DPIP) as the measurement tool. The transfer of electrons during photosynthesis reduces DPIP, changing it from blue to clear.
Materials 10 mL 4.5% Acetic Acid (Vinegar), C2H4O2 | 100 mL 0.5 M Sucrose Solution (cold!), C12H22O11 *You Must Provide | |
Pre-Lab Questions
1. Describe how the functional units for beta carotene, xanthophyll, chlorophyll A, and chlorophyll B are different. Be sure to identify the subunits that adhere to paper during chromatography.
2. Describe a technique for measuring photosynthetic rate.
3. Many deciduous trees have leaves which turn yellow in the fall. What do you suppose is happening in the leaves at the cellular and molecular level?
4. Chloroplasts and mitochondria are both are unusual in that they have double membranes and contain their own set of DNA. Can you think of any explanations for this observation?
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Solution: BIO Lab 20 Pre-Lab Questions (2015)