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Assignment 1Note: I need only two pages for discussion Environmental Monitoring Cleanroom (Microbiology).It is very important to focus on the discussion of the results in the order and connect them with pictures and also discuss the reason for the growth of bacteria in some places and samples while not grow in the samples and other places.Generally required to discuss all the results and should be first class.I will send you the methodology and results and I need only the discussionEnvironmental Monitoring (Clean Room)Methodology:1) One litter of SDA was prepared and poured into 22 petri dishes and 10 contact plates.2) One litter of TSA was prepared and poured into 22 petri dishes and 10 contact plates.3) A sit map plan was drawn, entry time was signed and the garb was worn to enter the clean room.4) Then, the following tests were done in the clean room: (A) Particulate contamination test:This test carried out by using the particle counter (ARTI particle counter) to find out the number and the size of particles in the clean room air. (B) Microbiological tests:1- Air sampling:Settle plates (Passive):Five pairs of agar plates (SDA and TSA) were left open for 30 minutes in five areas in clean room as shown in the site map plan (See Figure 1).Centrifugal air sampler (Active):agar plate of SDA and one agar plate of TSA were placed into the centrifugal air sampler to take air samples.2- Surface sampling:Contact plates:Five pairs of contact agar plates (SDA and TSA) were used in five areas in clean room as shown in the site map plan (See Figure 1).Swabs:Five pairs of agar plates (SDA and TSA) were used in five areas in clean room as shown in the site map plan (See Figure 1).ATP bioluminescence (Rapid method):One Biotrace swap was used to take a sample from the glove box. Then, the Biotrace swab was placed into the Unilite instrument to record RLU reading.5) The surfaces of the clean room were cleaned by using the appropriate klercide agent.6) Then, the steps 4 and 5 were repeated.7) The garb was taken off and the clean room was exited.8) The plates were incubated at 30-37C for a week.9) Gram stain was done for plates that has a growth bacteria.Results:(Table 2: The results of microbiological tests of clean room before cleaning)AgarsAir samplesSurface SamplePassiveActiveContactSwapsATPArea 1SDANo GrowthTSA: G+ (Cocci, Purpul) Fig4SDA: No GrowthNo GrowthNo Growth18 RLU Fig9TSAG+ (Cocci, Purpul) Fig3No GrowthNo GrowthArea 2SDANo GrowthNo GrowthNo GrowthTSANo GrowthNo GrowthNo GrowthArea 3SDANo GrowthNo GrowthNo GrowthTSANo GrowthNo GrowthNo GrowthArea 4SDANo GrowthNo GrowthNo GrowthTSANo GrowthNo GrowthNo GrowthArea 5SDANo GrowthNo GrowthNo GrowthTSANo GrowthNo GrowthG- (Cocci, Pink) Fig5(Table 4: The results of microbiological tests of clean room after cleaning)AgarsAir samplesSurface SamplePassiveActiveContactSwapsATPArea 1SDANo GrowthTSA: No GrowthSDA: No GrowthNo GrowthNo Growth12 RLU Fig10TSANo Growth No GrowthNo GrowthNo GrowthArea 2SDAG+ (Cocci, Purpul) Fig6No GrowthNo GrowthTSANo GrowthNo GrowthNo GrowthArea 3SDANo GrowthNo GrowthNo GrowthTSANo GrowthNo GrowthNo GrowthArea 4SDAG+ (Cocci, Purpul) Fig7No GrowthNo GrowthTSANo GrowthNo GrowthNo GrowthArea 5SDANo GrowthNo GrowthNo GrowthTSANo GrowthNo GrowthG+ (Cocci, Purpul) Fig8Discussion:Appendix:3163721900452343785142240Figure 1 (The site map plan)Figure 2 (ARTI Particle Counter / Before and Cleaning)102616019685Figure 3 (Gram stain G+, settle TSA plate for Area 1/ Before Cleaning)779780-570865Figure 4 (Gram stain G+, Active TSA by Air Centrifuge Sampler/ Before Cleaning)784860203835Figure 5 (Gram stain G-, Swap TSA plate for Area 5/ Before Cleaning)77978040640Figure 6 (Gram stain G+, settle SDA plate for Area 2/ After Cleaning)739140-354965kFgggFigure 7 (Gram stain G+, settle SDA plate for Area 4/ After Cleaning)766445144145-5365752478405Figure 9 (ATP reading / Before Cleaning) 00Figure 9 (ATP reading / Before Cleaning) 30073602478405Figure 10 (ATP reading/ After Cleaning)00Figure 10 (ATP reading/ After Cleaning)3127375594995-270510596265Figure 8 (Gram stain G+, Swap TSA plate for Area 5/ After Cleaning)

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Preview: a xxxxx deal xxxx you consider xxx procedure talked xxxxx We xxx xxx the xxxxxxxxxxx coming on xxxx the activities xxxx are xxxxxxx xx a xxx by day xxxxx by individuals xxxx is xx xxxxxxx practices xxxx eating or xxxx the things xx buy xxx xxx use xxx general cleanliness xxxxx affects the xxxx at xxxxx xx have xxxxxxxxx growth and xxxxxxxxxxx When an xxxxxxxxxx stays xx x room xxxx has no xxxxxxx to the xxxxxxx standards, xxxx xxxxx to xxxxxxxx we would xxx to it xxxx we xxxx xxxx some xxxxxx regarding the xxxxxxx as a xxxxx We xxxx xxx that xxxx of bacteria xxxx develop and, xxxxxxxxxx giving xxx xxxxxxxx family xx arena that xxxxx help them xx.....
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