The citric acid cycle enzyme, fumarase

Question # 00623337 Posted By: dr.tony Updated on: 11/29/2017 01:02 PM Due on: 11/29/2017
Subject Biology Topic Biochemistry Tutorials:
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1) The citric acid cycle enzyme, fumarase, catalyzes the conversion of fumarate to malate: (15 points)

Fumarate + H2O à Malate

The turnover number, kcat, for fumarase is 800/sec. The Km of fumarase for its substrate is 5uM.

(a) In an experiment using 2 nM of fumarase, what is Vmax?

(b) The cellular concentration of fumarate is 47.5 uM. What is V0 when [fumarate] = 47.5 uM?

(c) What is the catalytic efficiency of fumarase?

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